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overlap extension pcr  (New England Biolabs)


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    New England Biolabs overlap extension pcr
    Overlap Extension Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/overlap+extension+pcr/Q5+High-Fidelity+PCR+Kit/pm41388072-177-15-23
    Average 99 stars, based on 2258 article reviews
    overlap extension pcr - by Bioz Stars, 2026-08
    99/100 stars

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    Construction of tandem gene duplications. ( A ) <t>PCR</t> <t>fragment</t> with a selectable marker (“R”) at the novel junction. The fragments typically carry 75 base genome homologies at the ends. ( B ) Recombination with a replicating chromosome creates a gene duplication which carries the selectable marker R (usually for gentamicin resistance).
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    Construction of tandem gene duplications. ( A ) PCR fragment with a selectable marker (“R”) at the novel junction. The fragments typically carry 75 base genome homologies at the ends. ( B ) Recombination with a replicating chromosome creates a gene duplication which carries the selectable marker R (usually for gentamicin resistance).

    Journal: mBio

    Article Title: Short tandem gene duplications as potential agents of genetic memory

    doi: 10.1128/mbio.02011-25

    Figure Lengend Snippet: Construction of tandem gene duplications. ( A ) PCR fragment with a selectable marker (“R”) at the novel junction. The fragments typically carry 75 base genome homologies at the ends. ( B ) Recombination with a replicating chromosome creates a gene duplication which carries the selectable marker R (usually for gentamicin resistance).

    Article Snippet: A DNA fragment corresponding to the deletion was constructed by extension overlap PCR and introduced into plasmid pU1 ( ) by USER cloning (New England Biolabs).

    Techniques: Marker